Membrane-associated Ribosomes in Mitochondria of Neurospora Crassa
نویسندگان
چکیده
The poky (mi-1) strain of Neurospora crassa is a and the mass ratio of large to small subunit RNA useful test organism in which to evaluate methods is -10 :1 (1) . Nonetheless, mitochondrial protein for isolating mitochondrial ribosomes actively synthesis can be observed in poky (2) and cytoengaged in protein synthesis . In poky mitochondria chrome oxidase which is in part synthesized by there is a deficiency of small ribosomal subunits Neurospora mitochondria (3, 4) is present at a level
منابع مشابه
Sites of biosynthesis of outer and inner membrane proteins of Neurospora crassa mitochondria.
Outer and inner membranes of Neurospora crassa mitochondria were separated by the combined swelling, shrinking, sonication procedure. Membranes were characterized by electron microscopy and by marker enzyme activities. A red carotenoid pigment was found to be concentrated in the outer membrane. The inner mitochondrial membrane was resolved into about 20 protein bands on polyacrylarnide gel elec...
متن کاملTransport of cytoplasmically synthesized proteins into the mitochondria in a cell free system from Neurospora crassa.
Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic ribosomes are transferred into the mitochondrial fraction. The relative amounts of proteins which are transferred in vitro are comparable to those transferred in whole cells. Cycloheximide and puromyc...
متن کاملThe 73 S ribosome of Neurospora crassa is the native mitochondrial ribosome.
The effect of the inclusion of EDTA and of heparin, in media used in the isolation of mitochondria, on the mitochindrial ribosome has been investigated. 1. Mitochondria isolated from Neurospora crassa in the presence of EDTA contain only a single type of monomeric ribosome, viz. 73 S. 2. Mitochondria isolated in the presence of Mg 2÷ contain both 79-S and 73-S monomeric ribosomes. The heterogen...
متن کاملDefective production of mitochondrial ribosomes in the poky mutant of Neurospora crassa.
A strain of Neurospora crassa containing a cytoplasmic mutation (poky) affecting mitochondrial function is shown to be deficient in small ribosomal subunits in the mitochondrion during the exponential growth phase. In the stationary growth phase, small subunits are more abundant and are present in mitochondrial ribosomal monomers. This change can be correlated with the return of mitochondrial c...
متن کاملPrimary structure and mitochondrial import in vitro of the 20.9 kDa subunit of complex I from Neurospora crassa.
The 20.9 kDa subunit of NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa is a nuclear-coded component of the hydrophobic arm of the enzyme. We have determined the primary structure of this subunit by sequencing a full-length cDNA and a cleavage product of the isolated polypeptide. The deduced protein sequence is 189 amino acid residues long and contains a putative membrane-span...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 59 شماره
صفحات -
تاریخ انتشار 1973